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Development of a real-time PCR assay for the detection of Clonorchis sinensis DNA in gallbladder bil

更新时间:2012-12-21 14:52:53 作者: 来源:

Development of a real-time PCR assay for the detection of Clonorchis sinensis DNA in gallbladder bile and stone samples from patients with cholecystolithiasis

 

Tie Qiao & Pei-Ming Zheng & Rui-Hong Ma &

 

Xiao-Bing Luo & Zhen-Liang Luo


Received: 25 February 2012 /Accepted: 23 May 2012 /Published online: 6 June 2012

 

# Springer-Verlag 2012

Abstract High prevalence of cholecystolithiasis in parts of East Asia has been postulated to be associated with Clonorchis sinensis infection. This study describes the development of a TaqMan-based real-time PCR assay for the detection of C. sinensis DNA in gallbladder bile and stone samples from patients with cholecystolithiasis. Primers and probe targeting the cytochrome c oxidase subunit 1 gene of mitochondrial DNA proved to be highly specific for C. sinensis and did not amplify other related heterogeneous DNA samples. The detec-tion limit of this assay was 0.1 pg of adult C. sinensis genomic DNA. All of the egg-positive samples determined by micros-copy yielded positive results by real-time PCR assay and that genetic testing of gallbladder stones using real-time PCR was considered as the most effective means for assessing C. sinensis infection status. This assay not only contributes to a greater understanding of stone pathogenesis but also benefits patients with cholecystolithiasis by facilitating effective diagnosis, treat-ment, and relapse prevention.


 

【原文链接】

Development of a real-time PCR assay for the detection of Clonorchis sinensis DNA in gallbladder bile and stone samples from patients with cholecystolithiasis.pdf
文件类型: .pdf 70b488860cde9a4cbec110c9cf66846d.pdf (753.72 KB)